Forensic use of Y-chromosome DNA: a general overview

The male-specific part of the human Y chromosome is widely used in forensic DNA analysis, particularly in cases where standard autosomal DNA profiling is not informative. A Y-chromosomal gene fragment is applied for inferring the biological sex of a crime scene trace donor. Haplotypes composed of Y-chromosomal short tandem repeat polymorphisms (Y-STRs) are used to characterise paternal lineages of unknown male trace donors, especially suitable when males and females have contributed to the same trace, such as in sexual assault cases. Y-STR haplotyping applied in crime scene investigation can (i) exclude male suspects from involvement in crime, (ii) identify the paternal lineage of male perpetrators, (iii) highlight multiple male contributors to a trace, and (iv) provide investigative leads for finding unknown male perpetrators. Y-STR haplotype analysis is employed in paternity disputes of male offspring and other types of paternal kinship testing, including historical cases, as well as in special cases of missing person and disaster victim identification involving men. Y-chromosome polymorphisms are applied for inferring the paternal bio-geographic ancestry of unknown trace donors or missing persons, in cases where autosomal DNA profiling is uninformative. In this overview, all different forensic applications of Y-chromosome DNA are described. To illustrate the necessity of forensic Y-chromosome analysis, the investigation of a prominent murder case is described, which initiated two changes in national forensic DNA legislation both covering Y-chromosome use, and was finally solved via an innovative Y-STR dragnet involving thousands of volunteers after 14 years. Finally, expectations for the future of forensic Y-chromosome DNA analysis are discussed

Grassland Renovation and Consequences for Nutrient Management

Sward degradation is a serious threat to the functioning of grassland and the provision of ecosystem services. Renovation measures are frequently applied in order to restore degraded swards. However, the success is highly variable and substantial tradeoffs are often found following renovation such as among agronomic and environmental services. Starting from a general classification of renovation measures the paper investigates the processes induced by renovation that lead to a change of the vegetation and that affect carbon and nitrogen fluxes. These processes are strongly interrelated and dependent on site, climate and management condition as well as on the time scale. The more an existing and degraded sward is deliberately disturbed prior to a renovation measure, e.g. by ploughing, the larger will be the vegetation change, the potential yield and quality advantage but also the risk of soil organic carbon release and nitrogen emissions to the environment. Such effects are unlikely to maintain in the longer term. This demonstrates that the renovation of swards is always the second best solution if there is the opportunity to avoid degradation by a proper grassland utilization

Nitrogen Dynamics Following the Break-up of Grassland on Three Different Sandy Soils

Nitrogen (N) is accumulated under grassland depending on factors such as soil type, management, and fertiliser input. Break-up of grassland stimulates mineralisation of organic N and may lead to increased soil mineral N and leaching losses (Lloyd, 1992). The objective of this study was to find out how site factors, e.g. soil, previous management and sward age, the following crop and the new level of N fertiliser affect the amount of inorganic N in autumn and the preceding mineralisation processes when grassland is ploughed in spring